Periostin Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54036r-bf594-100uL |
| Product Name | Periostin Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Human Perilipin-1 aa 400 to the C-terminus |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10631 |
| Swiss Prot | Q15063 |
| Synonyms | Periostin; Osteoblast-specific factor 2; PN; OSF-2; POSTN; OSF2; PDLPOSTN. |
| Background | Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide-linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta and alpha(V)beta integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor _ 1(TGF _ 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:20-50 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Periostin Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54036R-BF594) validated for, and at what dilution?
bsm-54036R-BF594 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:20-50); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Periostin Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54036R-BF594) react with?
Tested reactivity: Human.
How should bsm-54036R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Periostin Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54036R-BF594)?
Human Perilipin-1 aa 400 to the C-terminus
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-54036R-BF594)?
AbBy Fluor-594 on bsm-54036R-BF594: excitation / emission 590nm/617nm.