CFH Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54051r-bf488-100uL |
| Product Name | CFH Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | Full length protein |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3075 |
| Swiss Prot | P08603 |
| Subcellular location | Secreted |
| Synonyms | Complement factor H, H factor 1, CFH, HF, HF1, HF2 |
| Background | A glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self-markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is CFH Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54051R-BF488) validated for, and at what dilution?
bsm-54051R-BF488 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CFH Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54051R-BF488) react with?
Tested reactivity: Human.
How should bsm-54051R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CFH Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54051R-BF488)?
Full length protein
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-54051R-BF488)?
AbBy Fluor-488 on bsm-54051R-BF488: excitation / emission 499nm/519nm.