Fascin (12A1) Monoclonal Antibody

$Lane 1: SH-SY5Y Cells; Lane 2: Human Brain; Lane 3: Rat Spleen; Lane 4: Mouse Testis; Lane 5: Mouse Brain; Lane 6: Mouse Spleen; Probed with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SH-SY5Y Cells; Lane 2: Human Brain; Lane 3: Rat Spleen; Lane 4: Mouse Testis; Lane 5: Mouse Brain; Lane 6: Mouse Spleen; Probed with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Hela cells with Fascin (12A1) Monoclonal Antibody (bsm-54075R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54075R
Product Name	Fascin (12A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Monoclonal
Clone #	12A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6624
Swiss Prot	Q16658
Synonyms	Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55, FSCN1, FAN1, HSN, SNL
Background	Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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