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IKB epsilon Recombinant Antibody

Lane 1: Human U937 cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Hela cell lysates; Lane 6: Human HepG2 cell lysates probed with NFKBIE Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human U937 cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Hela cell lysates; Lane 6: Human HepG2 cell lysates probed with NFKBIE Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKB epsilon Monoclonal Antibody, Unconjugated (bsm-54094R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-54094r-100uL
Product Name IKB epsilon Recombinant Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human IKB epsilon protein
Clonality Recombinant
Clone # 7A4
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4794
Swiss Prot O00221
Subcellular location Cytoplasm
Synonyms IKBE; IKBE_HUMAN; NFKBIE; NF-kappa-BIE; I-kappa-B-epsilon (IkB-E | IkB-epsilon | IkappaBepsilon); NFKB inhibitor epsilon; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon; NF-kappa-B inhibitor epsilon
Background The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF

Frequently asked questions

Which applications is IKB epsilon Recombinant Antibody (bsm-54094R) validated for, and at what dilution?

bsm-54094R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IKB epsilon Recombinant Antibody (bsm-54094R) react with?

Tested reactivity: Human.

How should bsm-54094R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IKB epsilon Recombinant Antibody (bsm-54094R)?

Recombinant human IKB epsilon protein

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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