NFKBIE (7A4) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with NFKBIE (7A4) Monoclonal Antibody (bsm-54094R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with NFKBIE (7A4) Monoclonal Antibody (bsm-54094R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: THP-1 Cells; Lane 2: Jurkat Cells; Lane 3: Hela Cells; Probed with NFKBIE (7A4) Monoclonal Antibody (bsm-54094R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: THP-1 Cells; Lane 2: Jurkat Cells; Lane 3: Hela Cells; Probed with NFKBIE (7A4) Monoclonal Antibody (bsm-54094R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54094R
Product Name	NFKBIE (7A4) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Monoclonal
Clone #	7A4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4794
Swiss Prot	O00221
Synonyms	NF-kappa-B inhibitor epsilon; I-kappa-B-epsilon; NF-kappa-BIE; IkB-E; IkB-epsilon; IKB epsilon; IkappaBepsilon; NFKBIE; NFKB inhibitor epsilon
Background	The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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