phospho-MLKL (Ser345) Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54104r-bf680 |
| Product Name | phospho-MLKL (Ser345) Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL |
| Modification Site | Ser345 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 74568 |
| Swiss Prot | Q9D2Y4 |
| Synonyms | Phospho-MLKL (S345); pMLKL Ser345; Mixed lineage kinase domain-like protein, Mlkl |
| Background | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-MLKL (Ser345) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-54104R-BF680) validated for, and at what dilution?
bsm-54104R-BF680 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MLKL (Ser345) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-54104R-BF680) react with?
Tested reactivity: Mouse.
How should bsm-54104R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MLKL (Ser345) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-54104R-BF680)?
Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-54104R-BF680)?
AbBy Fluor-680 on bsm-54104R-BF680: excitation / emission 679nm/702nm.