Ferritin Heavy Chain (5B5) Monoclonal Antibody

$IF(ICC) staining with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Brain tissue incubated with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Brain tissue incubated with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Ferritin Heavy Chain (5B5) Monoclonal Antibody (bsm-54108R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54108R
Product Name	Ferritin Heavy Chain (5B5) Monoclonal Antibody
Applications	FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human Ferritin Heavy Chain
Clonality	Monoclonal
Clone #	5B5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2495
Swiss Prot	P02794
Synonyms	Ferritin heavy chain; Cell proliferation-inducing gene 15 protein; Ferritin heavy chain; N-terminally processed; Ferritin H subunit; FTH1, FTH, FTHL6; FHC; HFE5; PLIF; PIG15
Background	Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Application Dilution
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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