Angiopoietin 2 (4G2) Monoclonal Antibody

$Lane 1: TF-1 Cells; Lane 2: Human Liver; Lane 3: Human Placenta; Probed with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: TF-1 Cells; Lane 2: Human Liver; Lane 3: Human Placenta; Probed with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with Angiopoietin 2 (4G2) Monoclonal Antibody (bsm-54113R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54113R
Product Name	Angiopoietin 2 (4G2) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Angiopoietin 2 aa 200-350
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	285
Swiss Prot	O15123
Synonyms	Angiopoietin-2; ANG-2; ANGPT2.
Background	Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400