TFAM (16A1) Monoclonal Antibody

$Lane 1: Hela Cells; Probed with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Probed with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100 in SK-Br-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100 in SK-Br-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated TFAM (16A1) Monoclonal Antibody (bsm-54118R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54118R
Product Name	TFAM (16A1) Monoclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human mtTFA aa 50-150
Clonality	Monoclonal
Clone #	16A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	7019
Swiss Prot	Q00059
Synonyms	Transcription factor A, mitochondrial; Mitochondrial transcription factor 1; Transcription factor 6; Transcription factor 6-like 2; mtTFA; MtTF1; TCF-6; TFAM; TCF6; TCF6L2.
Background	Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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