PFKFB3 (1G2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54120R-100uL |
| Product Name | PFKFB3 (1G2) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human PFKFB3 aa 300 to the C-terminus |
| Clonality | Monoclonal |
| Clone # | 1G2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 5209 |
| Swiss Prot | Q16875 |
| Synonyms | 6-phosphofructo-2-kinase/fructose-2 6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2; 6-phosphofructo-2-kinase; Fructose-2 6-bisphosphatase; 6PF-2-K/Fru-2 6-P2ase 3; PFK/FBPase 3; PFKFB3. |
| Background | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) validated for, and at what dilution?
bsm-54120R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54120R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PFKFB3 (1G2) Monoclonal Antibody (bsm-54120R)?
Recombinant protein within human PFKFB3 aa 300 to the C-terminus