25% Off Flow Cytometry Antibodies - Use Code: "EggyIggy2024" - Valid 3/29-4/5/24 for U.S. customers only

PDCD6IP (9B3) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54129R
Product Name PDCD6IP (9B3) Monoclonal Antibody
Applications WB, FCM, IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human ALIX aa 1-150
Clonality Monoclonal
Clone # 9B3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID 10015
Swiss Prot Q8WUM4
Synonyms Programmed cell death 6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95; PDCD6-interacting protein; PDCD6IP; AIP1; ALIX; KIAA1375; HP95; DRIP4.
Background Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP. By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier. PDCD6IP has also found to be involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400