DNA Ligase IV Recombinant Antibody

$Lane 1: Human MCF-7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Ramos cell lysates; Lane 6: Human HepG2 cell lysates probed with DNA Ligase IV Monoclonal Antibody, Unconjugated (bsm-54131R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Ramos cell lysates; Lane 6: Human HepG2 cell lysates probed with DNA Ligase IV Monoclonal Antibody, Unconjugated (bsm-54131R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-54131r
Product Name	DNA Ligase IV Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human DNA Ligase IV aa 500-700
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3981
Swiss Prot	P49917
Subcellular location	Nucleus
Synonyms	DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4.
Background	Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100