DNA Ligase IV (2D9) Monoclonal Antibody

$Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Jurkat Cells; Probed with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Jurkat Cells; Probed with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Human MCF-7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Ramos cell lysates; Lane 6: Human HepG2 cell lysates probed with DNA Ligase IV Monoclonal Antibody, Unconjugated (bsm-54131R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Ramos cell lysates; Lane 6: Human HepG2 cell lysates probed with DNA Ligase IV Monoclonal Antibody, Unconjugated (bsm-54131R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54131R
Product Name	DNA Ligase IV (2D9) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human DNA Ligase IV aa 500-700
Clonality	Monoclonal
Clone #	2D9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3981
Swiss Prot	P49917
Subcellular location	Nucleus
Synonyms	DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4.
Background	Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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