RAPGEF3 (3D1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54133R |
| Product Name | RAPGEF3 (3D1) Monoclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Epac1 aa 700-900 |
| Clonality | Monoclonal |
| Clone # | 3D1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 10411 |
| Swiss Prot | O95398 |
| Synonyms | Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; EPAC 1; cAMP-GEFI; RAPGEF3; CGEF1; EPAC; EPAC1; bcm910; HSU79275. |
| Background | Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R) validated for, and at what dilution?
bsm-54133R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54133R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAPGEF3 (3D1) Monoclonal Antibody (bsm-54133R)?
Recombinant protein within human Epac1 aa 700-900