Galectin 1 (9H8) Monoclonal Antibody

$IF(ICC) staining with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Stomach tissue incubated with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Stomach tissue incubated with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of NIH-3T3 cells with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: HL-60 Cells; Lane 2: Human Skeletal Muscle; Probed with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HL-60 Cells; Lane 2: Human Skeletal Muscle; Probed with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54137R
Product Name	Galectin 1 (9H8) Monoclonal Antibody
Applications	WB FCM IHC-P IF(IHC-P) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Galectin 1 aa 1-150
Clonality	Monoclonal
Clone #	9H8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3956
Swiss Prot	P09382
Synonyms	Galectin-1; 14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1; Gal-1; HLBP14; LGALS1.
Background	The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200