OPA1 (9A7) Monoclonal Antibody

Flow cytometric analysis of SH-SY5Y cells with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Lane 1: Mouse Brain; Lane 2: A431 Cells; Probed with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Brain; Lane 2: A431 Cells; Probed with OPA1 (9A7) Monoclonal Antibody (bsm-54144R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54144R
Product Name	OPA1 (9A7) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human OPA1
Clonality	Monoclonal
Clone #	9A7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	4976
Swiss Prot	O60313
Subcellular location	Mitochondrion
Synonyms	Dynamin-like 120 kDa protein mitochondrial; Optic atrophy protein 1; OPA1; KIAA0567.
Background	OPA1 is a cause of optic atrophy type 1. OPA1 is mostly expressed in the mitochondrial biogenesis. OPA1 is a cause of optic atrophy type 1. OPA1 is mostly expressed In retina but can also be expressed in brain, testis, heart and skeletal muscles. This dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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