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OPA1 (9A7) Monoclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54144r-cy3-100uL
Product Name OPA1 (9A7) Monoclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source C terminal Human OPA1
Clonality Monoclonal
Clone # 9A7
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4976
Swiss Prot O60313
Subcellular location Mitochondrion
Synonyms Dynamin-like 120 kDa protein mitochondrial; Optic atrophy protein 1; OPA1; KIAA0567.
Background OPA1 is a cause of optic atrophy type 1. OPA1 is mostly expressed in the mitochondrial biogenesis. OPA1 is a cause of optic atrophy type 1. OPA1 is mostly expressed In retina but can also be expressed in brain, testis, heart and skeletal muscles. This dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission.
Application Dilution
WB 1:300-5000
IF 1:50-200

Frequently asked questions

Which applications is OPA1 (9A7) Monoclonal Antibody, Cy3 Conjugated (bsm-54144R-Cy3) validated for, and at what dilution?

bsm-54144R-Cy3 is validated for WB (1:300-5000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OPA1 (9A7) Monoclonal Antibody, Cy3 Conjugated (bsm-54144R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-54144R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OPA1 (9A7) Monoclonal Antibody, Cy3 Conjugated (bsm-54144R-Cy3)?

C terminal Human OPA1

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54144R-Cy3)?

Cy3 on bsm-54144R-Cy3: excitation / emission 512,550nm/570,615nm.