Aldolase A (2C9) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in SK-BR-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in SK-BR-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Lane 1: Zebrafish Tissue; Probed with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:200 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Zebrafish Tissue; Probed with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:200 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: A549 Cells; Lane 2: Hela Cells; Lane 3: Mouse Liver; Lane 4: Rat Liver; Lane 5: Rat Spleen; Probed with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A549 Cells; Lane 2: Hela Cells; Lane 3: Mouse Liver; Lane 4: Rat Liver; Lane 5: Rat Spleen; Probed with Aldolase A (2C9) Monoclonal Antibody (bsm-54146R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-54146R
Product Name	Aldolase A (2C9) Monoclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Aldolase aa 1-150
Clonality	Monoclonal
Clone #	2C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	226
Swiss Prot	P04075
Synonyms	Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDOA; ALDA; GSD12; HEL-S-87p.
Background	This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. In addition, may also function as a scaffolding protein. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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