Rad51 (11D1) Monoclonal Antibody

$Lane 1: Hela Cells; Lane 2: Human Kidney; Lane 3: Mouse Testis; Probed with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: Human Kidney; Lane 3: Mouse Testis; Probed with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Testis tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Testis tissue incubated with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Jurkat cells with Rad51 (11D1) Monoclonal Antibody (bsm-54150R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: NIH\/3T3, 3: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 37 kDa\r\nObserved MW: 37 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: NIH\/3T3, 3: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 37 kDa\r\nObserved MW: 37 kDa

$Tissue: Human testis\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54150R\r\n$

Tissue: Human testis\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54150R\r\n

$Tissue: Rat testis\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54150R\r\n$

Tissue: Rat testis\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54150R\r\n

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with Rad51 Monoclonal Antibody, Unconjugated (bsm-54150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with Rad51 Monoclonal Antibody, Unconjugated (bsm-54150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54150R
Product Name	Rad51 (11D1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Rad51 aa 1-150
Clonality	Monoclonal
Clone #	11D1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5888
Swiss Prot	Q06609
Synonyms	DNA repair protein RAD51 homolog 1; RAD51 homolog A; HsRAD51; hRAD51; RAD51; RAD51A; RECA; BRCC5; FANCR; MRMV2; HsT16930.
Background	Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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