CBX3 (4B4) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Hela cells with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: PC-12 Cells; Lane 2: MCF-7 Cells; Probed with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-12 Cells; Lane 2: MCF-7 Cells; Probed with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54154R
Product Name	CBX3 (4B4) Monoclonal Antibody
Applications	WB FCM IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human CBX3 aa 1-150
Clonality	Monoclonal
Clone #	4B4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	11335
Swiss Prot	Q13185
Subcellular location	Nucleus
Synonyms	HP1 gamma; Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; Modifier 2 protein; CBX3; HP1-GAMMA; HP1Hs-gamma.
Background	Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400