ACTN4 (6A1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54157R-100uL |
| Product Name | ACTN4 (6A1) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human alpha Actinin 4 aa 1-150 |
| Clonality | Monoclonal |
| Clone # | 6A1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 81 |
| Swiss Prot | O43707 |
| Synonyms | Alpha-actinin-4; Non-muscle alpha-actinin 4; ACTN4; FSGS; FSGS1; ACTININ-4; Focal Segmental Glomerulosclerosis 1 |
| Background | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) validated for, and at what dilution?
bsm-54157R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54157R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ACTN4 (6A1) Monoclonal Antibody (bsm-54157R)?
Recombinant protein within human alpha Actinin 4 aa 1-150