SAMHD1 (16C6) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54174R-100uL |
| Product Name | SAMHD1 (16C6) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide within human SAMHD1 aa 600 to the C-terminus |
| Clonality | Monoclonal |
| Clone # | 16C6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 25939 |
| Swiss Prot | Q9Y3Z3 |
| Subcellular location | Nucleus |
| Synonyms | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1; Dendritic cell-derived IFNG-induced protein; Monocyte protein 5; SAM domain and HD domain-containing protein 1; dNTPase; DCIP; MOP-5; SAMHD1; MOP5. |
| Background | SAMHD1 may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) validated for, and at what dilution?
bsm-54174R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) react with?
Tested reactivity: Human.
How should bsm-54174R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R)?
Synthetic peptide within human SAMHD1 aa 600 to the C-terminus