SAMHD1 (16C6) Monoclonal Antibody

$Lane 1: THP-1 Cells; Probed with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: THP-1 Cells; Probed with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of K563 cells with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54174R
Product Name	SAMHD1 (16C6) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human SAMHD1 aa 600 to the C-terminus
Clonality	Monoclonal
Clone #	16C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	25939
Swiss Prot	Q9Y3Z3
Subcellular location	Nucleus
Synonyms	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1; Dendritic cell-derived IFNG-induced protein; Monocyte protein 5; SAM domain and HD domain-containing protein 1; dNTPase; DCIP; MOP-5; SAMHD1; MOP5.
Background	SAMHD1 may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Search our catalog of hundreds of thousands of products