SDHB Recombinant Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54180r-percp-Cy5.5-100uL |
| Product Name | SDHB Recombinant Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | Recombinant protein within human SDHB aa 1-150 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6390 |
| Swiss Prot | P21912 |
| Subcellular location | Mitochondrion |
| Synonyms | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, Ip, SDHB, SDH, SDH1 |
| Background | Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SDHB Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-54180R-PERCP-CY5-5) validated for, and at what dilution?
BSM-54180R-PERCP-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SDHB Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-54180R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-54180R-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SDHB Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-54180R-PERCP-CY5-5)?
Recombinant protein within human SDHB aa 1-150
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-54180R-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-54180R-PERCP-CY5-5: excitation / emission 482nm/694nm.