HDAC9 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54186r-bf594-100uL |
| Product Name | HDAC9 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Recombinant protein within human HDAC9 aa 1-150 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9734 |
| Swiss Prot | Q9UKV0 |
| Subcellular location | Nucleus |
| Synonyms | Histone deacetylase 9; Histone deacetylase 7B; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HD9; HD7; HD7b; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR. |
| Background | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is HDAC9 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54186R-BF594) validated for, and at what dilution?
bsm-54186R-BF594 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HDAC9 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54186R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54186R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HDAC9 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54186R-BF594)?
Recombinant protein within human HDAC9 aa 1-150
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-54186R-BF594)?
AbBy Fluor-594 on bsm-54186R-BF594: excitation / emission 590nm/617nm.