Staufen (8C8) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54191R-100uL |
| Product Name | Staufen (8C8) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Staufen aa 400-550 |
| Clonality | Monoclonal |
| Clone # | 8C8 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 6780 |
| Swiss Prot | O95793 |
| Subcellular location | Cytoplasm, Endoplasmic reticulum |
| Synonyms | Double-stranded RNA-binding protein Staufen homolog 1; PPP1R150; STAU1; STAU. |
| Background | STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Staufen (8C8) Monoclonal Antibody (bsm-54191R) validated for, and at what dilution?
bsm-54191R is validated for WB (1:300-5000); IF(ICC) (1:50-200); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Staufen (8C8) Monoclonal Antibody (bsm-54191R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54191R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Staufen (8C8) Monoclonal Antibody (bsm-54191R)?
Recombinant protein within human Staufen aa 400-550