SPTAN1 (4C8) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: NIH-3T3 Cells; Lane 4: Rat Brain; Lane 5: Mouse Brain; Probed with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: NIH-3T3 Cells; Lane 4: Rat Brain; Lane 5: Mouse Brain; Probed with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54194R
Product Name	SPTAN1 (4C8) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Alpha Fodrin aa 2300-2450
Clonality	Monoclonal
Clone #	4C8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6709
Swiss Prot	Q13813
Synonyms	Spectrin alpha chain non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain; Spectrin non-erythroid alpha subunit; NEAS; SPTA2;
Background	Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. SPTAN1 is an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants. SPTAN1, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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