TPT1 (7C1) Monoclonal Antibody

$Lane 1: Mouse Placenta; Lane 2: Mouse Testis; Lane 3: Hela Cells; Lane 4: A431 Cells; Lane 5: MCF-7 Cells; Probed with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Placenta; Lane 2: Mouse Testis; Lane 3: Hela Cells; Lane 4: A431 Cells; Lane 5: MCF-7 Cells; Probed with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of THP-1 cells with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54198R
Product Name	TPT1 (7C1) Monoclonal Antibody
Applications	WB FCM IHC-P IF(IHC-P) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein corresponding to human TCTP 1-150
Clonality	Monoclonal
Clone #	7C1
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	7178
Swiss Prot	P13693
Subcellular location	Cytoplasm
Synonyms	tumor protein translationally-controlled 1; Translationally-controlled tumor protein; Fortilin; Histamine-releasing factor; p23; TCTP; HRF; TPT1; p02.
Background	TPT1 is a regulator of cellular growth and proliferation. Its mRNA is highly structured and contains an oligopyrimidine tract (5'-TOP) in its 5' untranslated region that functions to repress its translation under quiescent conditions. The protein is involved in a variety of cellular pathways, including apoptosis, protein synthesis and cell division. It binds to and stabilizes microtubules, and removal of this protein through phosphorylation is required for progression through mitotic and meiotic cell divisions. This gene is known to play a role in carcinogenesis, and is upregulated in some cancer cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200