Nuclear Matrix Protein p84 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Nuclear Matrix Protein p84 monoclonal antibody, unconjugated (bsm-54202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Nuclear Matrix Protein p84 monoclonal antibody, unconjugated (bsm-54202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54202r
Product Name	Nuclear Matrix Protein p84 Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Nuclear Matrix Protein p84
Clonality	Recombinant
Clone #	6C1
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9984
Swiss Prot	Q96FV9
Subcellular location	Cytoplasm, Nucleus
Synonyms	DFNA86; HPR1; P84; P84N5; 3110002N20Rik; NMP-84; tho1; LRRGT00070; THOC1_HUMAN; THOC1; Nuclear matrix protein p84 (p84N5); hTREX84; THOC1_MOUSE; Nuclear matrix protein p84; THOC1_RAT; Liver regeneration-related protein LRRG175; THO complex subunit 1; THO complex 1
Background	THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy.
Application Dilution
WB	1:500-1000