NCK1 Recombinant Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-54207r-pe-100ul |
| Product Name | NCK1 Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCK1 |
| Clonality | Recombinant |
| Clone # | 7C2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4690 |
| Swiss Prot | P16333 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NCK; NCKalpha; nck-1; 6330586M15Rik; D230010O13Rik; NCK1_HUMAN; NCK1; Cytoplasmic protein NCK1; NCK adapter protein 1 (Nck-1); SH2/SH3 adapter protein NCK-alpha; NCK1_MOUSE; NCK adaptor protein 1 |
| Background | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is NCK1 Recombinant Antibody, PE Conjugated (bsm-54207R-PE) validated for, and at what dilution?
bsm-54207R-PE is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCK1 Recombinant Antibody, PE Conjugated (bsm-54207R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat.
How should bsm-54207R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCK1 Recombinant Antibody, PE Conjugated (bsm-54207R-PE)?
KLH conjugated synthetic peptide derived from human NCK1
What are the excitation and emission wavelengths of the PE conjugate (bsm-54207R-PE)?
PE on bsm-54207R-PE: excitation / emission 496,564nm/578nm.