PTBP1 (7D3) Monoclonal Antibody

$Lane 1: K562 Cells; Lane 2: Jurkat Cells; Lane 3: Mouse Colon; Probed with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2: Jurkat Cells; Lane 3: Mouse Colon; Probed with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54208R
Product Name	PTBP1 (7D3) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human PTBP1 1-200
Clonality	Monoclonal
Clone #	7D3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5725
Swiss Prot	P26599
Subcellular location	Nucleus
Synonyms	Polypyrimidine tract-binding protein 1; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; PTB; hnRNP I; PTBP1.
Background	PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). PTBP1 a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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