MAD2L1 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54219r-100uL |
| Product Name | MAD2L1 Recombinant Antibody |
| Applications |
WB
IHC-P
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Mad2L1 aa 1-150 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 4085 |
| Swiss Prot | Q13257 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Mitotic spindle assembly checkpoint protein MAD2A; Mitotic arrest deficient 2-like protein 1; HsMAD2; MAD2-like protein 1; MAD2L1; MAD2. |
| Background | Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAD2L1 Recombinant Antibody (bsm-54219R) validated for, and at what dilution?
bsm-54219R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAD2L1 Recombinant Antibody (bsm-54219R) react with?
Tested reactivity: Human.
How should bsm-54219R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise MAD2L1 Recombinant Antibody (bsm-54219R)?
Recombinant protein within human Mad2L1 aa 1-150