KAT2B (2C1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54229R-100uL |
| Product Name | KAT2B (2C1) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human PCAF aa 100-300 |
| Clonality | Monoclonal |
| Clone # | 2C1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 8850 |
| Swiss Prot | Q92831 |
| Subcellular location | Nucleus |
| Synonyms | Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; Histone acetylase PCAF; P/CAF; KAT2B; PCAF. |
| Background | Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4 and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Also acetylates spermidine. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is KAT2B (2C1) Monoclonal Antibody (bsm-54229R) validated for, and at what dilution?
bsm-54229R is validated for WB (1:300-5000); IF(ICC) (1:50-200); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KAT2B (2C1) Monoclonal Antibody (bsm-54229R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54229R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KAT2B (2C1) Monoclonal Antibody (bsm-54229R)?
Recombinant protein within human PCAF aa 100-300