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NR1D1 Recombinant Antibody

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NR1D1 antibody (bsm-54240R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NR1D1 antibody (bsm-54240R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NR1D1 Monoclonal Antibody, Unconjugated (bsm-54240R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P
  • Flow-Cyt

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-54240R-100uL
Product Name NR1D1 Recombinant Antibody
Applications
WB IHC-F IF IHC-P FLOW-CYT
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human NR1D1 aa 500 to the C-terminus
Clonality Monoclonal
Clone # 6D1
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID 9572
Swiss Prot P20393
Subcellular location Cytoplasm, Nucleus
Synonyms Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1; NR1D1; EAR1; HREV; THRAL.
Background This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular, this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes.
Application Dilution
WB 1:500-1000
IHC-F 1:400-800
IF 1:400-800
IHC-P 1:100-500
Flow-Cyt 1:50-100

Frequently asked questions

Which applications is NR1D1 Recombinant Antibody (bsm-54240R) validated for, and at what dilution?

bsm-54240R is validated for WB (1:500-1000); IHC-F (1:400-800); IF (1:400-800); IHC-P (1:100-500); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NR1D1 Recombinant Antibody (bsm-54240R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-54240R be stored?

Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NR1D1 Recombinant Antibody (bsm-54240R)?

Recombinant protein within human NR1D1 aa 500 to the C-terminus

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