NR1D1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-54240r-rbitc-100ul |
| Product Name | NR1D1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Recombinant protein within human NR1D1 aa 500 to the C-terminus |
| Clonality | Monoclonal |
| Clone # | 6D1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9572 |
| Swiss Prot | P20393 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1; NR1D1; EAR1; HREV; THRAL. |
| Background | This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular, this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. |
| Application Dilution | |
| WB | 1:500-1000 |
| IF | 1:400-800 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is NR1D1 Recombinant Antibody, RBITC Conjugated (bsm-54240R-RBITC) validated for, and at what dilution?
bsm-54240R-RBITC is validated for WB (1:500-1000); IF (1:400-800); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NR1D1 Recombinant Antibody, RBITC Conjugated (bsm-54240R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-54240R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NR1D1 Recombinant Antibody, RBITC Conjugated (bsm-54240R-RBITC)?
Recombinant protein within human NR1D1 aa 500 to the C-terminus
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-54240R-RBITC)?
RBITC on bsm-54240R-RBITC: excitation / emission 570nm/595nm.