PFKM Recombinant Antibody

$Lane 1: PC-3M Cells; Probed with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-3M Cells; Probed with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Thyroid Gland tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Thyroid Gland tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of SH-SY5Y cells with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54245R
Product Name	PFKM Recombinant Antibody
Applications	WB FCM IHC-P IF(IHC-P) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Fructose 6 Phosphate Kinase 1-200
Clonality	Monoclonal
Clone #	14C3
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5213
Swiss Prot	P08237
Subcellular location	Cytoplasm
Synonyms	ATP-dependent 6-phosphofructokinase muscle type; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; Phosphohexokinase; ATP-PFK; PFK-M; PFK-A; PFKM; PFKX.
Background	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200