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SET Recombinant Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SET) monoclonal Antibody, unconjugated (bsm-54264R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SET) monoclonal Antibody, unconjugated (bsm-54264R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SET antibody (bsm-54264R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SET antibody (bsm-54264R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with SET monoclonal antibody, unconjugated (bsm-54264R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with SET monoclonal antibody, unconjugated (bsm-54264R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SET Monoclonal Antibody, Unconjugated (bsm-54264R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-54264r-100uL
Product Name SET Recombinant Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic peptide within Human SET aa 200 to the C-terminus
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID 6418
Swiss Prot Q01105
Subcellular location Cytoplasm, Nucleus, Endoplasmic reticulum
Synonyms Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase, PHAPII; Phosphatase 2A inhibitor I2PP2A; Template-activating factor I; IGAAD; I-2PP2A; TAF-I.
Background Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Application Dilution
WB 1:500-2000
IHC-P 1:100-200
IHC-F 1:100-200
IF 1:100-200
Flow-Cyt 1:50-100
ICC/IF 1:50-200

Frequently asked questions

Which applications is SET Recombinant Antibody (bsm-54264R) validated for, and at what dilution?

bsm-54264R is validated for WB (1:500-2000); IHC-P (1:100-200); IHC-F (1:100-200); IF (1:100-200); Flow-Cyt (1:50-100); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SET Recombinant Antibody (bsm-54264R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-54264R be stored?

Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise SET Recombinant Antibody (bsm-54264R)?

Synthetic peptide within Human SET aa 200 to the C-terminus

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