Mast Cell Chymase (1G11) Monoclonal Antibody

$Lane 1: Rat Spleen; Lane 2: Mouse Spleen; Probed with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Spleen; Lane 2: Mouse Spleen; Probed with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Rat

Overview
Catalog #	bsm-54265R
Product Name	Mast Cell Chymase (1G11) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Mast Cell Chymase aa 50-200
Clonality	Monoclonal
Clone #	1G11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	1215
Swiss Prot	P23946
Subcellular location	Secreted
Synonyms	Chymase 1; Alpha-chymase; Mast cell protease I; CMA1; CYH; CYM.
Background	Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast Cell Chymase, also known as CMA1 or MCT1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19 amino acid signal peptide, an acidic 2 amino acid propeptide and a 226 amino acid catalytic domain. Mast Cell Chymase is a chymotryptic serine proteinase which is a member of the peptidase family S1. Expressed in mast cells, Mast Cell Chymase is associated with the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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