RBPJ (1D4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54268R-100uL |
| Product Name | RBPJ (1D4) Monoclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human RBPJK aa 1-200 |
| Clonality | Monoclonal |
| Clone # | 1D4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 3516 |
| Swiss Prot | Q06330 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; Renal carcinoma antigen NY-REN-30; RBP-J; RBP-JK; RBPJ; IGKJRB; IGKJRB1; RBPJK; RBPSUH |
| Background | Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is RBPJ (1D4) Monoclonal Antibody (bsm-54268R) validated for, and at what dilution?
bsm-54268R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RBPJ (1D4) Monoclonal Antibody (bsm-54268R) react with?
Tested reactivity: Human.
How should bsm-54268R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RBPJ (1D4) Monoclonal Antibody (bsm-54268R)?
Recombinant protein within human RBPJK aa 1-200