PSMB9 (1G9) Monoclonal Antibody

$Lane 1: Mouse Spleen; Lane 2: U937 Cells; Lane 3: Mouse Colon; Probed with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Spleen; Lane 2: U937 Cells; Lane 3: Mouse Colon; Probed with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Daudi cells with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54270R
Product Name	PSMB9 (1G9) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human PSMB9 aa 180 to the C-terminus
Clonality	Monoclonal
Clone #	1G9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5698
Swiss Prot	P28065
Subcellular location	Cytoplasm, Nucleus
Synonyms	Proteasome subunit beta type-9; Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Proteasome chain 7; Proteasome subunit beta-1i; Really interesting new gene 12 protein; PSMB9; LMP2; PSMB6i; RING12; Proteasome 20S LMP2
Background	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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