Alpha Synuclein Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54277r-biotin-100uL |
| Product Name | Alpha Synuclein Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Recombinant full length Human protein |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 6622 |
| Swiss Prot | P37840 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane |
| Synonyms | Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; NACP; PARK1; PARK4 |
| Background | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity |
| Application Dilution | |
| WB | 1:1000-2000 |
| IHC-P | IHC-P1:200-800 |
| IHC-F | IHC-F1:200-800 |
Frequently asked questions
Which applications is Alpha Synuclein Recombinant Antibody, Biotin Conjugated (bsm-54277R-Biotin) validated for, and at what dilution?
bsm-54277R-Biotin is validated for WB(WB) (1:1000-2000); IHC-P(IHC-P) (1:200-800); IHC-F(IHC-F) (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Alpha Synuclein Recombinant Antibody, Biotin Conjugated (bsm-54277R-Biotin) react with?
Tested reactivity: Human, Rat.
How should bsm-54277R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Alpha Synuclein Recombinant Antibody, Biotin Conjugated (bsm-54277R-Biotin)?
Recombinant full length Human protein