Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54281R-100uL |
| Product Name | Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Cytochrome P450 Reductase aa 1-150 |
| Clonality | Monoclonal |
| Clone # | 4F7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 5447 |
| Swiss Prot | P16435 |
| Subcellular location | Endoplasmic reticulum |
| Synonyms | NADPH cytochrome P450 reductase; CPR; P450R; POR; CYPOR; Cytochrome p450 Reductase |
| Background | Cytochrome P450 Oxidoreductase is an endoplasmic reticulum membrane oxidoreductase with a FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. It can also provide electron transfer to heme oxygenase and cytochrome B5. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) validated for, and at what dilution?
bsm-54281R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54281R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R)?
Recombinant protein within human Cytochrome P450 Reductase aa 1-150