BCL11A Recombinant Antibody

$Lane 1: Daudi Cells; Lane 2: U937 Cells; Probed with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cells; Lane 2: U937 Cells; Probed with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Jurkat cells with BCL11A (4A11) Monoclonal Antibody (bsm-54284R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54284R
Product Name	BCL11A Recombinant Antibody
Applications	WB FCM IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Ctip1 aa 400-600
Clonality	Monoclonal
Clone #	4A11
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	53335
Swiss Prot	Q9H165
Subcellular location	Nucleus
Synonyms	B-cell lymphoma/leukemia 11A; B-cell CLL/lymphoma 11A; COUP-TF-interacting protein 1; Ecotropic viral integration site 9 protein homolog; Zinc finger protein 856; BCL-11A; EVI-9; BCL11A; CTIP1; EVI9; KIAA1809; ZNF856
Background	Transcription factor associated with the BAF SWI/SNF chromatin remodeling complex. Repressor of fetal hemoglobin (HbF) level. Involved in brain development. Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. Essential factor in lymphopoiesis required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400