Calsequestrin-1 (8G1) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of C2C12 cells with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54285R
Product Name	Calsequestrin-1 (8G1) Monoclonal Antibody
Applications	FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Calsequestrin 1 aa 100-250
Clonality	Monoclonal
Clone #	8G1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	844
Swiss Prot	P31415
Subcellular location	Mitochondrion, Endoplasmic reticulum
Synonyms	Calsequestrin 1; Calmitine; Calsequestrin skeletal muscle isoform; CASQ1; CASQ; PDIB1; VMCQA
Background	The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
Application Dilution
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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