Calsequestrin-1 (8G1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54285R-100uL |
| Product Name | Calsequestrin-1 (8G1) Monoclonal Antibody |
| Applications |
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Calsequestrin 1 aa 100-250 |
| Clonality | Monoclonal |
| Clone # | 8G1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 844 |
| Swiss Prot | P31415 |
| Subcellular location | Mitochondrion, Endoplasmic reticulum |
| Synonyms | Calsequestrin 1; Calmitine; Calsequestrin skeletal muscle isoform; CASQ1; CASQ; PDIB1; VMCQA |
| Background | The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium. |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) validated for, and at what dilution?
bsm-54285R is validated for IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54285R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R)?
Recombinant protein within human Calsequestrin 1 aa 100-250