NCOA1 (2G9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54289R-100uL |
| Product Name | NCOA1 (2G9) Monoclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human KAT13A/ SRC1 aa 450-700 |
| Clonality | Monoclonal |
| Clone # | 2G9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 8648 |
| Swiss Prot | Q15788 |
| Subcellular location | Nucleus |
| Synonyms | Nuclear receptor coactivator 1; Class E basic helix-loop-helix protein 74; Protein Hin-2; RIP160; Renal carcinoma antigen NY-REN-52; Steroid receptor coactivator 1; NCoA-1; bHLHe74; SRC-1; NCOA1; BHLHE74; SRC1 |
| Background | Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) validated for, and at what dilution?
bsm-54289R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) react with?
Tested reactivity: Human.
How should bsm-54289R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCOA1 (2G9) Monoclonal Antibody (bsm-54289R)?
Recombinant protein within human KAT13A/ SRC1 aa 450-700