NCOA1 (2G9) Monoclonal Antibody

$Lane 1: K562 Cells; Probed with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Probed with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Thyroid Gland tissue incubated with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Thyroid Gland tissue incubated with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with NCOA1 (2G9) Monoclonal Antibody (bsm-54289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54289R
Product Name	NCOA1 (2G9) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human KAT13A/ SRC1 aa 450-700
Clonality	Monoclonal
Clone #	2G9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	8648
Swiss Prot	Q15788
Subcellular location	Nucleus
Synonyms	Nuclear receptor coactivator 1; Class E basic helix-loop-helix protein 74; Protein Hin-2; RIP160; Renal carcinoma antigen NY-REN-52; Steroid receptor coactivator 1; NCoA-1; bHLHe74; SRC-1; NCOA1; BHLHE74; SRC1
Background	Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

Search our catalog of hundreds of thousands of products