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Gelsolin Recombinant Antibody, RBITC Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54290r-rbitc
Product Name Gelsolin Recombinant Antibody, RBITC Conjugated
Applications
WB IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source Recombinant human Gelsolin protein
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2934
Swiss Prot P06396
Subcellular location Cytoplasm, Secreted
Synonyms Actin depolymerizing factor; ADF; AGEL; Amyloidosis Finnish type; Brevin; DKFZp313L0718; GSN; GELS_HUMAN; Actin-depolymerizing factor.
Background Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Application Dilution
WB 1:500-2000
IF 1:50-200
Flow-Cyt 1:50-100

Frequently asked questions

Which applications is Gelsolin Recombinant Antibody, RBITC Conjugated (bsm-54290R-RBITC) validated for, and at what dilution?

bsm-54290R-RBITC is validated for WB (1:500-2000); IF (1:50-200); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Gelsolin Recombinant Antibody, RBITC Conjugated (bsm-54290R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-54290R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Gelsolin Recombinant Antibody, RBITC Conjugated (bsm-54290R-RBITC)?

Recombinant human Gelsolin protein

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-54290R-RBITC)?

RBITC on bsm-54290R-RBITC: excitation / emission 570nm/595nm.