Huntingtin Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54305r-bf647-100uL |
| Product Name | Huntingtin Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | Recombinant protein within human Huntingtin aa 1-150 |
| Immunogen Range | 1-150/3142 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3064 |
| Swiss Prot | P42858 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Huntingtin; Huntington disease protein; HD protein; HTT; IT15; LOMARS |
| Background | Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. Huntingtin may play a role in microtubule-mediated transport or vesicle function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Huntingtin Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-54305R-BF647) validated for, and at what dilution?
bsm-54305R-BF647 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Huntingtin Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-54305R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54305R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Huntingtin Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-54305R-BF647)?
Recombinant protein within human Huntingtin aa 1-150 Immunogen range: 1-150/3142.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-54305R-BF647)?
AbBy Fluor-647 on bsm-54305R-BF647: excitation / emission 650nm/665nm.