STIP1 (2H5) Monoclonal Antibody

$Lane 1: Mouse Testis; Lane 2: Rat Testis; Probed with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Testis; Lane 2: Rat Testis; Probed with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of K562 cells with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54308R
Product Name	STIP1 (2H5) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein orresponding to human STIP1 aa 1-150
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10963
Swiss Prot	P31948
Subcellular location	Cytoplasm, Nucleus
Synonyms	Stress-induced-phosphoprotein 1; Hsc70/Hsp90-organizing protein; Renal carcinoma antigen NY-REN-11; Transformation-sensitive protein IEF SSP 3521; STI1; Hop; STIP1
Background	Stress-induced-phosphoprotein 1 (STI1) functions as a co-chaperone for HSP70 and HSP90 during heat shock response. STI1 exists as either a monomer or a dimer, and this conformational flexibility facilitates its function in organizing HSP70/HSP90. HSP90 acts as an ATPase, and requires the recruitment of client proteins and proper conformation to function. STI1 acts as a "scaffold" for client protein recruitment to the relaxed, ADP-bound conformation of HSP90, thus suppressing ATP turnover during the loading phase and allowing proper function.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400