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Desmoglein 1 Recombinant Antibody, RBITC Conjugated

Applications

  • WB
  • IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54326r-rbitc-100uL
Product Name Desmoglein 1 Recombinant Antibody, RBITC Conjugated
Applications
WB IF
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source Recombinant protein within human DSG1 aa 900 to the C-terminus
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1828
Swiss Prot Q02413
Subcellular location Cell membrane
Synonyms Desmoglein-1; Cadherin family member 4; Desmosomal glycoprotein 1; Pemphigus foliaceus antigen; DG1; DGI; DSG1; CDHF4
Background Desmogleins (dsgs) are type I membrane proteins that are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions. DSG1 is a component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Application Dilution
WB 1:500-2000
IF 1:100-500

Frequently asked questions

Which applications is Desmoglein 1 Recombinant Antibody, RBITC Conjugated (bsm-54326R-RBITC) validated for, and at what dilution?

bsm-54326R-RBITC is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-54326R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Desmoglein 1 Recombinant Antibody, RBITC Conjugated (bsm-54326R-RBITC)?

Recombinant protein within human DSG1 aa 900 to the C-terminus

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-54326R-RBITC)?

RBITC on bsm-54326R-RBITC: excitation / emission 570nm/595nm.