Cubilin Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54329r-biotin-100uL |
| Product Name | Cubilin Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Recombinant protein within C-terminal human Cubilin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 8029 |
| Swiss Prot | O60494 |
| Subcellular location | Lysosome, Cell membrane, Endosome |
| Synonyms | CUBN; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor; IFCR |
| Background | Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
Frequently asked questions
Which applications is Cubilin Recombinant Antibody, Biotin Conjugated (bsm-54329R-Biotin) validated for, and at what dilution?
bsm-54329R-Biotin is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cubilin Recombinant Antibody, Biotin Conjugated (bsm-54329R-Biotin) react with?
Tested reactivity: Human.
How should bsm-54329R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cubilin Recombinant Antibody, Biotin Conjugated (bsm-54329R-Biotin)?
Recombinant protein within C-terminal human Cubilin