CD46 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54335r-bf594-100uL |
| Product Name | CD46 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Synthetic peptide within human CD46 aa 50-100 |
| Clonality | Monoclonal |
| Clone # | 4C3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4179 |
| Swiss Prot | P15529 |
| Synonyms | Membrane cofactor protein; TLX; Trophoblast leukocyte common antigen; CD46; MCP; MIC10; AHUS2; |
| Background | Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CD46 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54335R-BF594) validated for, and at what dilution?
bsm-54335R-BF594 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD46 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54335R-BF594) react with?
Tested reactivity: Human.
How should bsm-54335R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD46 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-54335R-BF594)?
Synthetic peptide within human CD46 aa 50-100
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-54335R-BF594)?
AbBy Fluor-594 on bsm-54335R-BF594: excitation / emission 590nm/617nm.