SNRPA (2A1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54338R-100uL |
| Product Name | SNRPA (2A1) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IHC
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein corresponding to N-terminal human U1A. |
| Clonality | Monoclonal |
| Clone # | 2A1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 6626 |
| Swiss Prot | P09012 |
| Subcellular location | Nucleus |
| Synonyms | U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1-A; U1A; SNRPA |
| Background | Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is SNRPA (2A1) Monoclonal Antibody (bsm-54338R) validated for, and at what dilution?
bsm-54338R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SNRPA (2A1) Monoclonal Antibody (bsm-54338R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54338R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SNRPA (2A1) Monoclonal Antibody (bsm-54338R)?
Recombinant protein corresponding to N-terminal human U1A.