CENPC1 Monoclonal Antibody

$Lane 1: K562 Cells; Probed with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Probed with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

$Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with CENPC (3A7) Monoclonal Antibody (bsm-54360R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Flow cytometric analysis of K562 cells with CENPC (3A7) Monoclonal Antibody (bsm-54360R)at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Lane 1: Human MOLT-4 cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with CENPC Monoclonal Antibody, Unconjugated (bsm-54360R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54360R
Product Name	CENPC1 Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human CENPC1 protein
Clonality	Monoclonal
Clone #	3A7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1060
Swiss Prot	Q03188
Subcellular location	Nucleus
Synonyms	CENP-C 1; CENP-C; CENPC_HUMAN; CENPC1; Centromere autoantigen C; Centromere autoantigen C1; Centromere protein C 1; ICEN7; Interphase centromere complex protein 7; MIF2.
Background	Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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